| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | KIMBROUGH, Arnezsia | SR | STEAM Academy at McCluer South-B | 2:30.91 | Unverified: |
| 2 | EDWARDS, Justine | SR | Gateway High School | 2:36.42 | Verified |
| 3 | GRIGGS, Dinajia | SO | East St. Louis (Senior) HS | 2:39.00 | Unverified: |
| 4 | BURNS, Le'nikei | JR | East St. Louis (Senior) HS | 2:50.10 | Verified |
| 5 | COOPER, Katrina | SO | McCluer High School | 3:15.00 | Unverified: |
| 6 | CHANEY, Anandra | 9 | Normandy High School | 3:46.45 | Verified |
| 7 | BAUM-SCHOLLE, Lucy | 9 | Metro High School | NT | Unverified: |
