| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Metro High School | A | Macon-Ford | Fuchs | Christian | Kamara | NT | Unverified: |
| 3 | McCluer North High School | A | 50.07 | Unverified: | ||||
| 4 | STEAM Academy at McCluer South-B | A | 49.02 | Verified | ||||
| 5 | East St. Louis (Senior) HS | A | Lampley | Graham | Jones | Eastern | 49.10 | Unverified: |
| 6 | Riverview Gardens High School | A | 58.57 | Verified |
