| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Riverview Gardens High School | A | NT | |||||
| 2 | Metro High School | A | Fuchs | Kamara | Kovarik | Macon-Ford | NT | Unverified: |
| 3 | McCluer North High School | A | 1:51.42 | Verified | ||||
| 4 | STEAM Academy at McCluer South-B | A | 1:43.58 | Unverified: | ||||
| 5 | East St. Louis (Senior) HS | A | Graham | Jones | Lampley | Lampley | 1:46.10 | Unverified: |
| 6 | Lift for Life Academy Charter Hi | A | NT | Verified | ||||
| 7 | Normandy High School | A | Howard | Brownlee | Williams | Howard | NT | Unverified: |
