| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | Rockwood Summit High School | A | Mounger | BUTCHKO | Lackey | Krobath | 3:40.61 | Verified |
| 4 | St. Louis University High School | A | Pashea | Byrne | Piening | Wingo | 3:36.85 | Unverified: McCullough-Douglas Invitational |
| 5 | DeSmet Jesuit High School | A | Gray | Hentges | Byas | Thomas | 3:40.00 | Unverified: |
| 6 | Lafayette (Wildwood) High School | A | 3:44.00 | Unverified: |
