| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 2 | MURASKI, Elias | JR | Kirkwood High School | 49.42 | Verified |
| 3 | PARKER, Keshon | JR | St. Louis University High School | 45.66 | Verified |
| 4 | GARNER, Pernell | SR | Lafayette (Wildwood) High School | 43.00 | Unverified: |
| 5 | GRENINGER, Conner | JR | St. Louis University High School | 44.12 | Verified |
| 6 | BROWN, Mason | JR | Rockwood Summit High School | 46.94 | Verified |
| 7 | GJELVIK, Ethan | SO | Kirkwood High School | 50.10 | Unverified: |
