| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | NAES, Connor | SO | Kirkwood High School | 51.30 | Unverified: |
| 2 | HOEFT, MATTHEW | 9 | Lafayette (Wildwood) High School | 48.00 | Unverified: |
| 3 | JOVCESKI, Isaac | 9 | Lafayette (Wildwood) High School | 47.00 | Unverified: |
| 5 | ALCORN, Cameron | 9 | Rockwood Summit High School | 46.44 | Verified |
| 6 | VEHIGE, MATTHEW | 9 | Lafayette (Wildwood) High School | 47.50 | Unverified: |
| 7 | KOSTOS, Maximus | SO | Kirkwood High School | 51.20 | Unverified: |
| 8 | PRICHER, Maxwell | SO | Kirkwood High School | 51.40 | Unverified: |
