| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 2 | BYRNE, Aidan | SR | St. Louis University High School | 53.54 | Unverified: |
| 3 | KROBATH, Drew | JR | Rockwood Summit High School | 51.64 | Verified |
| 4 | WINGO, Ryan | 9 | St. Louis University High School | 51.11 | Unverified: |
| 5 | HAMPTON, Malik | JR | Lafayette (Wildwood) High School | 51.34 | Unverified: |
| 7 | HENTGES, Peter | JR | DeSmet Jesuit High School | 54.54 | Verified |
