| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Rockwood Summit High School | A | Winkler | Lackey | Emmenegger | Talleur | 48.01 | Verified |
| 4 | DeSmet Jesuit High School | A | Stewart | Gray | Thomas | Mayes | 44.40 | Unverified: |
| 6 | St. Louis University High School | A | Bailey | Chaffee | Baker | Brooks | 46.02 | Unverified: |
