| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | Lafayette (Wildwood) High School | A | 48.60 | Unverified: | ||||
| 4 | Kirkwood High School | A | Hassan | Welch | Harris | Smith | 48.00 | Unverified: |
| 5 | Rockwood Summit High School | A | Horton | Rohan | Wallace | Vu | 48.01 | Verified |
| 6 | DeSmet Jesuit High School | A | Redd | Sharp | Key | Johnson | 48.74 | Verified |
