| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Jennings High School | A | NT | Verified | ||||
| 2 | Priory High School | A | Roxas | McMillan | Mercer | Schnurbusch | 3:41.43 | Verified |
| 3 | Whitfield School | A | Bobo | Milgie | Simon | Lee | 3:34.86 | Verified |
| 4 | Christian Brothers College High | A | Herrick | Wunderlich | Werner | Hamer | 3:40.49 | Verified |
| 5 | Miller Career Academy | A | Croft | Harlan | Gross | Collins | 4:21.91 | Verified |
