| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Priory High School | A | Singer | Glarner | Mercer | Fort | 46.82 | Verified |
| 3 | Christian Brothers College High | A | Woods | Smith | McClellan | Love | 44.15 | Unverified: |
| 4 | Miller Career Academy | A | Johnson | Slayton | Gleghorn | Williams | 46.21 | Verified |
| 5 | Jennings High School | A | NT | Verified |
