| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Hannibal High School | B | Erskine | Turner | Caldwell | Culp | 4:06.31 | Unverified: |
| 2 | Louisiana High School | A | Black | Greene | Hutt | Chatman | NT | Verified |
| 3 | Palmyra High School | A | Miller | Bogue | Hicks | Haerr | 3:51.89 | Verified |
| 4 | Monroe City High School | A | Talton | Martinez | Countryman | Hays | NT | Verified |
| 5 | Bowling Green High School | A | Korte | Hearn | Griffith | Gamm | 3:51.48 | Verified |
| 6 | Hannibal High School | A | Nichols | Tweedy | Hess | Ramage | 3:52.43 | Verified |
| 7 | South Shelby High School | A | Hammond | Friesz | Ratliff | Stevenson | 4:00.00 | Unverified: Macon Open |
