| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Louisiana High School | A | Bradley | Harrison | Tredway | Murray | 5:02.44 | Verified |
| 3 | Bowling Green High School | A | Gamm | Charlton | Mcmillen | Perry | 4:44.77 | Verified |
| 4 | Palmyra High School | A | White | Sublette | Sheputis | Loman | 4:29.09 | Verified |
| 5 | South Shelby High School | A | Wegman | Patterson | Hunolt | Gaines | 4:37.20 | Verified |
| 6 | Hannibal High School | A | Dorsey | Scott | Griffen | Bryant | 4:52.79 | Verified |
| 7 | Clark County High School | A | Brunk | St.Clair | Hess | WEAVER | NT | Unverified: |
