| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | STRAUS, Delaney | JR | Marion County High School | 5:21.45 | Verified |
| 2 | BUSCHLING, Grace | JR | Clark County High School | 5:36.18 | Verified |
| 3 | DORSEY, Jocelyn | 9 | Hannibal High School | 5:40.45 | Verified |
| 4 | HUNOLT, Summer | SO | South Shelby High School | 6:37.77 | Verified |
| 5 | RUFENER, Tadara | SO | South Shelby High School | 7:26.52 | Verified |
| 6 | WADDELL, Abbie | JR | Bowling Green High School | 7:36.07 | Verified |
| 7 | GARD, Lydia | JR | Palmyra High School | 7:36.19 | Verified |
| 8 | FRANKE, Peyton | SO | Clark County High School | NT | Unverified: |
| 9 | JOHNSON, Mia | 9 | Clark County High School | NT | Unverified: |
| 10 | STUPAVSKY, Isabella | SO | Monroe City High School | NT | Unverified: |
| 11 | OBRIEN, Jerorgia | SO | Palmyra High School | NT | Unverified: |
