| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Palmyra High School | A | Janes | Reid | Markley | Snyder | 1:56.25 | Verified |
| 2 | Louisiana High School | A | Black | Monjaraz | Rick) Tan | McBride | 1:45.72 | Verified |
| 3 | Bowling Green High School | A | Korte | Bryant | Fosdick | Bryant | 1:38.60 | Verified |
| 4 | Monroe City High School | A | Talton | Talton | Jones | Hays | 1:36.17 | Verified |
| 5 | Hannibal High School | A | Williams | Reading | Bergheger | Clubine | 1:36.55 | Unverified: Hannibal/Fulton Dual |
| 6 | South Shelby High School | A | Fenton | Wiseman | Hammond | Owens | 1:39.94 | Verified |
| 7 | Hannibal High School | B | 1:48.99 | Unverified: |
