| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Clark County High School | A | Brunk | Ross | St.Clair | Hess | NT | Unverified: |
| 2 | Louisiana High School | B | Campbell | Chatman | Sencenbaugh | Jenkins | 2:02.45 | Verified |
| 3 | Hannibal High School | A | Williams | Nichelson | Crane | Scott | 1:56.10 | Verified |
| 4 | Bowling Green High School | A | Charlton | Turner | Lewis | Gamm | 1:54.71 | Verified |
| 5 | Palmyra High School | A | Krigbaum | McBride | Mundy | White | 1:55.42 | Verified |
| 6 | South Shelby High School | A | Ziebarth | Curtis | Hendricks | Wegman | 1:59.91 | Verified |
| 7 | Louisiana High School | A | 2:02.45 | Verified |
