| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Palmyra High School | A | Reid | Janes | Markley | Snyder | 9:20.63 | Verified |
| 2 | Hannibal High School | A | Nichols | Hess | Ramage | Creech | 9:24.74 | Verified |
| 3 | Monroe City High School | A | Cheek | Crider | Griffin | Harlan | 9:25.03 | Verified |
| 4 | South Shelby High School | A | Stevenson | Burke | Blaine | Countryman | 9:33.45 | Verified |
