| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | PERRIMAN, Alexis | SO | Washington High School | NT | Unverified: |
| 2 | KROETER, Ella | SO | Washington High School | 1:05.00 | Unverified: |
| 3 | LAY, Alexis | 9 | Pacific High School | 53.00 | Unverified: |
| 4 | FIGAS, Ingrid | JR | Washington High School | 48.07 | Verified |
| 5 | CREGO, Kelsee | JR | Washington High School | 52.24 | Verified |
| 6 | KIMMINAU, Andrea | SR | St. Francis Borgia High School | 56.94 | Verified |
| 7 | JOHNSON, Avery | JR | Washington High School | NT | Unverified: |
| 8 | DELGADO, Emily | JR | New Haven High School | NT | Unverified: |
