| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | St. Clair Junior High School | A | Bethel | Bradshaw | Bartz | Nolan | NT | Unverified: |
| 3 | Pacific Middle School | A | Bernal | Clark | Martin | Wesloh | 4:51.04 | Verified |
| 4 | Sullivan Middle School | B | 4:20.00 | Unverified: | ||||
| 5 | Sullivan Middle School | A | Smith | Goly | Sparks | Beagle | 4:20.00 | Unverified: |
| 6 | Hermann Middle School | A | Gilbert | Kopp | Head | Austermann | 5:02.89 | Verified |
