| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Pacific Middle School | A | Adair | Cowsert | Robinson | Reed | 5:24.00 | Verified |
| 3 | Sullivan Middle School | A | Wall | Williams | Richardson | DeClue | 5:02.00 | Unverified: |
| 4 | Washington Middle School | A | Kiser | Mayer | Staples | James | 4:48.81 | Verified |
| 5 | Union Middle School | A | Cojocaru | Spiller | Spurgeon | Sykes | 4:58.07 | Verified |
| 6 | Owensville Middle School | A | Breedlove | Carey | Baguio | Gibson | 5:03.46 | Verified |
| 7 | St. Clair Junior High School | A | Cannon | Schatzler | Johnston | Shelton | NT | Unverified: |
