| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | LIMBERG, Ilene | 8 | Owensville Middle School | 2:44.41 | Verified |
| 2 | JOHANSON, Viola | 7 | Union Middle School | 2:46.94 | Verified |
| 3 | KISER, Presley | 8 | Washington Middle School | 2:50.26 | Verified |
| 4 | CANNON, Brooklyn | 8 | St. Clair Junior High School | 2:55.80 | Unverified: Owensville |
| 5 | SCHRECK, Cordelia | 7 | Union Middle School | 2:59.00 | Unverified: |
| 6 | STAPLES, Ava | 8 | Washington Middle School | 3:01.87 | Verified |
| 7 | BUSCH, Alley | 8 | Hermann Middle School | 3:01.91 | Verified |
| 8 | KANDLBINDER, Lauren | 8 | Owensville Middle School | 3:05.82 | Verified |
| 9 | MAJOR, Tabatha | 8 | St. Clair Junior High School | 3:07.00 | Unverified: Sullivan |
| 10 | REED, Mia | 8 | Union Middle School | 3:09.00 | Verified |
| 11 | ADAIR, Jenna | 8 | Pacific Middle School | 3:14.00 | Verified |
| 12 | GIBSON, Abbie | 8 | Owensville Middle School | 3:21.38 | Verified |
| 13 | REED, Bailey | 8 | Pacific Middle School | NT | Unverified: |
| 15 | VON BEHREN, Isabella | 8 | Washington Middle School | NT | Unverified: |
| 16 | DECLUE, Ava | 8 | Sullivan Middle School | NT | Unverified: |
| 17 | COWSERT, Michaela | 8 | Pacific Middle School | NT | Unverified: |
