| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | KEISER, Josie | 7 | Washington Middle School | 2:42.09 | Verified |
| 2 | UTHLAUT, Elaina | 7 | Hermann Middle School | 2:45.63 | Verified |
| 3 | LANE, Kealie | 7 | Hermann Middle School | 3:00.73 | Verified |
| 4 | DRYER, Grace | 7 | Pacific Middle School | 3:01.00 | Verified |
| 5 | GARGRAVE, Grace | 7 | Washington Middle School | 3:02.48 | Verified |
| 6 | GEHLERT, Hannah | 7 | Owensville Middle School | 3:05.83 | Verified |
| 7 | WINCHESTER, Sydnee | 7 | Washington Middle School | 3:12.73 | Verified |
| 8 | BAGUIO, Arianna | 7 | Owensville Middle School | 3:35.89 | Verified |
| 9 | CAREY, Aubree | 7 | Owensville Middle School | 3:37.02 | Verified |
| 10 | KOPPELMANN, Cali | 7 | Sullivan Middle School | NT | Unverified: |
| 11 | SNIDER, Taylor | 7 | Pacific Middle School | NT | Unverified: |
| 12 | VANDEGRIFFE, Iris | 7 | Sullivan Middle School | NT | Unverified: |
| 13 | DRAVES, Kaitlyn | 7 | St. Clair Junior High School | NT | Unverified: |
| 14 | CRONOVICH, Piper | 7 | Pacific Middle School | NT | Unverified: |
