| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Owensville Middle School | A | Winchester | Lebish | Reed | Blankenship | 1:04.67 | Verified |
| 3 | Hermann Middle School | A | Dodorico | Engemann | Schweich | Lane | 58.52 | Verified |
| 4 | Pacific Middle School | A | Dickey | Gebhard | Nemuth | Flood | 57.16 | Verified |
| 5 | Union Middle School | A | Sykes | Spiller | Koenigsfeld | Hall | 57.66 | Verified |
| 6 | Washington Middle School | A | Ruth | Newhouse | Ebenreck | Martin | 1:00.00 | Verified |
| 7 | St. Clair Junior High School | A | Shelton | Johnston | Sullivan | Schatzler | NT | Unverified: |
