| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | Washington Middle School | A | Hill | Bass | Carmean | Kitchen | 1:00.00 | Verified |
| 4 | Pacific Middle School | A | Bearden | Edmonds | Newton | Groom | 57.16 | Verified |
| 5 | Hermann Middle School | A | Kahler | Walker | Schutt | Hughes | 58.52 | Verified |
| 6 | Sullivan Middle School | A | Vandegriffe | Elias | Koppelmann | Hood | NT | Unverified: |
