| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Pacific Middle School | A | Bailey | Lawson | Denny | Dailey | 2:02.28 | Verified |
| 3 | Washington Middle School | A | Bina | Brune | Howard | Figas | 1:51.21 | Verified |
| 4 | Union Middle School | A | Sulschi | Burns | Sinclair | Schrader | 1:44.07 | Verified |
| 5 | Sullivan Middle School | A | Smith | Sanders | Goly | Sparks | 1:48.27 | Unverified: |
| 6 | St. Clair Junior High School | A | Nolan | Stanfill | Bethel | James | 1:53.11 | Unverified: Pacific |
| 7 | Owensville Middle School | A | Hawkins | Kramme | Lottmann | Connor | 2:15.84 | Verified |
