| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Pacific Middle School | A | Dickey | Gebhard | Nemuth | Flood | NT | Unverified: |
| 3 | Washington Middle School | A | Ruth | Newhouse | Ebenreck | James | 2:08.72 | Verified |
| 4 | Union Middle School | A | Sykes | Spiller | Koenigsfeld | Hall | 2:04.57 | Unverified: Union Middle School Meet |
| 5 | Owensville Middle School | A | Gregory | Comparato | Lebish | Vandegriffe | 2:07.19 | Verified |
| 6 | Hermann Middle School | A | Christian | Schulte | Schweich | Busch | 2:10.00 | Unverified: |
| 7 | St. Clair Junior High School | A | Shelton | Sullivan | Schatzler | Johnston | NT | Unverified: |
