| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | Hermann Middle School | A | McGarrah | Bentley | Engemann | Berkemeyer | NT | Unverified: |
| 4 | Washington Middle School | A | Hill | Craven | Highley | Hensley | 2:08.72 | Verified |
| 5 | Owensville Middle School | A | Reynolds | Lampkin | Scheer | McDaniel | 2:12.19 | Unverified: |
| 6 | Pacific Middle School | A | Howard | Stege | Groom | Douglas | NT | Unverified: |
