| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | HAMPTON, Annie | SR | Northwest Academy of Law | NT | Unverified: |
| 2 | CHRISTIAN, Maeta | JR | Metro High School | 1:07.52 | Verified |
| 3 | OUTLAW, aaniyah | SR | Soldan International Studies Hig | NT | Unverified: |
| 4 | EDWARDS, Justine | SR | Gateway High School | 1:10.68 | Verified |
| 5 | REED, Kennide | SO | Miller Career Academy | NT | Unverified: |
