| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Northwest Academy of Law | A | Foster | Clark | Robinson | Stinson | NT | Unverified: |
| 2 | Gateway High School | A | Cogio | Williams | Johnson | Muya | NT | Unverified: |
| 3 | Soldan International Studies Hig | A | Afolhyan | Edwards | Freeman | Johnson | 47.45 | Verified |
| 4 | Metro High School | A | Baum-Scholle | Dyer | Tiede | Appleton | NT | Unverified: |
| 5 | Roosevelt High School | A | Cotton | Davis | Lewis | Thornton | NT | Unverified: |
