| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Francis Howell North High School | A | Alexander | Stojanovic | Pollard | Miller | 3:53.50 | Verified |
| 3 | Marquette High School | A | Bush | Burke | Brown | CODY-JACKSON | 3:38.32 | Verified |
| 4 | Lafayette (Wildwood) High School | A | Hampton | Reed | Marshall | Garner | 3:32.29 | Unverified: |
| 5 | Parkway North High School | A | Logan | Weaver | Hauck | Parker | 3:32.40 | Verified |
| 6 | Parkway West High School | A | Campbell | Harris | Johnson | Moore | 3:42.00 | Unverified: Parkway Quad |
| 7 | Northwest (Cedar Hill) High Scho | A | Pearson | Godard | Robinson | Nicholls | 4:03.90 | Verified |
