| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Kirkwood High School | A | Kostos | Gjelvik | Pricher | Hassan | 4:05.00 | Unverified: SLUH Invitational |
| 3 | Parkway West High School | A | Feng | Hawver | Aiken-guerrier | Parini | 3:57.30 | Verified |
| 4 | Parkway North High School | A | Lau | Schrumpf | Jones | Stovall | 3:32.40 | Verified |
| 5 | Lafayette (Wildwood) High School | A | Compton | VEHIGE | Retych | Ems | 3:40.29 | Unverified: |
| 6 | Marquette High School | A | ANDERSON | GEORGE | MACHLUS | LODHI | 4:02.00 | Unverified: Lindbergh Flyers Boys Quad |
| 7 | Northwest (Cedar Hill) High Scho | A | Ash | Campbell | Keith | Bahr | 4:42.50 | Unverified: |
