| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | St. Joseph's Academy | A | Hanneken | Yelton | Grelle | Kelly | NT | Unverified: |
| 2 | Parkway North High School | A | Bevan | Koo | Ryan | Schrader | NT | Unverified: |
| 3 | Northwest (Cedar Hill) High Scho | A | Surratt | Ryan | Bertani | Skaggs | 4:29.00 | Unverified: Kirkwood Early Bird Quad |
| 4 | Eureka High School | A | Kleekamp | SCHNURBUSCH | DEAKIN | Grobe | 4:15.71 | Verified |
| 5 | Lafayette (Wildwood) High School | A | Derucki | Reed | White | Berry | 4:25.84 | Verified |
| 6 | Parkway West High School | A | Drake | Plass | Woodall | Sipp | 4:40.00 | Unverified: |
| 7 | STEAM Academy at McCluer South-B | A | Melvin | Melvin | Coleman | Reed | NT | Unverified: |
| 8 | Lindbergh High School | A | Bufalo | Page | Lorenz | Erpelding | NT | Unverified: |
