| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | WARE, Hope | SR | Lafayette (Wildwood) High School | 11:40.00 | Unverified: |
| 2 | BARNARD, Elissa | SO | Lafayette (Wildwood) High School | 11:40.00 | Unverified: |
| 3 | WHEELER, Karley | JR | Northwest (Cedar Hill) High Scho | 11:50.00 | Unverified: Northwest Time Trial |
| 4 | VOELKER, Kayla | SR | Eureka High School | 12:00.00 | Unverified: |
| 5 | ETCHASON, Alexandra | SO | Parkway West High School | 12:00.00 | Unverified: |
| 6 | POSEY, Ryley | SO | Northwest (Cedar Hill) High Scho | 12:00.00 | Unverified: Northwest Time Trial |
| 7 | GHORMLEY, Kylie | SR | Eureka High School | 12:51.32 | Verified |
| 8 | KLEIN, Margaret | JR | Kirkwood High School | 13:00.00 | Unverified: |
| 9 | CLANCY, Sophie | SO | Kirkwood High School | 13:00.00 | Unverified: |
| 10 | CASTELLANO, Julia | JR | St. Joseph's Academy | NT | Unverified: |
| 11 | JACKSON, Elizabeth | SO | Lindbergh High School | NT | Unverified: |
| 12 | GUENTHER, Cate | 9 | St. Joseph's Academy | NT | Unverified: |
| 13 | FORBES, Alyssa | SR | Lindbergh High School | NT | Unverified: |
