| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | STEAM Academy at McCluer South-B | A | NT | |||||
| 2 | Lindbergh High School | A | Halstead | Hertel | Thomas | Casaregola | NT | Unverified: |
| 3 | Lafayette (Wildwood) High School | A | Halter | Knysh | Singh | Brauch | 54.76 | Verified |
| 4 | Eureka High School | A | Dennis | DEAKIN | SEITZ | Grobe | 51.92 | Verified |
| 5 | Kirkwood High School | A | Wilkes | Burgett | Scully | Steele | 53.10 | Unverified: |
| 6 | Northwest (Cedar Hill) High Scho | A | Ryan | Orozco | Bertani | Skaggs | 55.40 | Unverified: Kirkwood Early Bird Quad |
