| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Roosevelt High School | A | Cotton | Davis | Lewis | Williams | NT | Verified |
| 2 | Miller Career Academy | A | Johnson | Slayton | Gross | Williams | 1:43.56 | Verified |
| 3 | Soldan International Studies Hig | A | 1:36.75 | Verified | ||||
| 4 | Gateway High School | A | 1:39.31 | Verified | ||||
| 5 | Metro High School | A | Fisher | Dawson | Custis | Cade | DQ | Verified |
| 6 | Vashon High School | A | Cole | Curry | Johnson | Morris | 1:50.32 | Verified |
