| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Roosevelt High School | A | Cotton | Thornton | Williams | Williams | NT | Verified |
| 2 | Vashon High School | A | Cole | Curry | Johnson | Morris | 48.00 | Verified |
| 3 | Miller Career Academy | A | Johnson | Slayton | Gleghorn | Williams | 46.21 | Verified |
| 4 | Soldan International Studies Hig | A | 46.34 | Verified | ||||
| 5 | Gateway High School | A | 48.16 | Verified | ||||
| 6 | Metro High School | A | Baum-Scholle | Dyer | Appleton | Tiede | 59.71 | Verified |
