| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Roosevelt High School | A | Clemons | MOOREHEAD | Siffray | Trevillion | 1:06.75 | Verified |
| 2 | Vashon High School | A | Ewing | Boyd | Taylor | Purnell | NT | Verified |
| 3 | Miller Career Academy | A | Fields | Flecher | Harris | 54.06 | Verified | |
| 4 | Soldan International Studies Hig | A | 54.62 | Verified | ||||
| 5 | Metro High School | A | Hudson | Jackson | Christian | Macon-Ford | 57.42 | Verified |
