| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | JENKINS, Morgan | Capitol Area Track Club 2 CAT | 2:37.00 | ||
| 2 | WRIGHT, Lucinda | KC FIRE Track Club | 2:40.00 | ||
| 3 | HURD, Olivia | Blues Track Club of Greater St | 2:50.00 | ||
| 4 | HILL, Jasmah | NEW LIFE GLIDERS | 2:55.00 | ||
| 5 | RUTHERFORD, Paeyton | Blues Track Club of Greater St | 2:59.32 | ||
| 6 | LEWIS, Marley | Blues Track Club of Greater St | 3:01.00 | ||
| 7 | BOEKHOUT, Noelle | Pirate Track Club | NT | ||
| 8 | HITCHCOCK, Karsyn | Johnson County Comets | NT | ||
| 9 | CREWS, Adriana | Unattached | NT | ||
| 10 | MILIUS, Addison | Blue Thunder Track Club | NT |
