| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | St. Joseph's Academy | A | Yelton | Grelle | Stryker | Pruett | 4:54.57 | Verified |
| 3 | Nerinx Hall High School | A | Medina | Brady | Schillinger | Jacknewitz | 4:24.39 | Verified |
| 4 | Cor Jesu Academy | A | Hinkamper | Steinbrueck | Sise | Reinlein | 4:46.03 | Unverified: |
| 5 | Villa Duchesne High School | A | Singer | Irvin | Gehm | Minner | 5:06.89 | Unverified: Villa Duchesne Dual Meet |
