| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Ursuline Academy | A | 1:01.35 | Verified | ||||
| 2 | Villa Duchesne High School | A | Hughes | Irvin | Wilson | Weingart | 56.53 | Unverified: Villa Duchesne Dual Meet |
| 3 | Incarnate Word Academy | A | Flowers | Moore | Moore | White | 52.51 | Verified |
| 4 | Cor Jesu Academy | A | Varghese | Schmidt | Graham | Wolf | 53.77 | Verified |
| 5 | Nerinx Hall High School | A | Johnson | Schirmer | Padberg | Ehnes | 58.89 | Verified |
