| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Ursuline Academy | A | NT | Unverified: | ||||
| 2 | Incarnate Word Academy | A | Anderson | Davis | Roberts | Moore | 2:04.15 | Verified |
| 3 | Cor Jesu Academy | A | Varghese | Hinkamper | Graham | Wolf | 1:54.77 | Verified |
| 4 | Nerinx Hall High School | A | Johnson | Padberg | Sanchez | Decker | 2:01.96 | Verified |
| 5 | Villa Duchesne High School | A | Minner | Acker | Pagen | Korte | 2:06.05 | Unverified: Villa Duchesne Dual Meet |
