| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Maplewood-Richmond Hts. High Sch | A | Marino | Morris | Scargall | Grawer | 9:25.89 | Verified |
| 2 | Maplewood-Richmond Hts. High Sch | B | Cummings | Lowery | Jacobs | Silsby | 10:20.00 | Unverified: Lutheran North Home Opener |
| 3 | Clayton High School | A | Lagesse | Patel | Cohn | Mysorekar | NT | Unverified: |
| 4 | Valley Park High School | A | Donahue | Bloemker | Sauceda | Sears | NT | Unverified: |
| 5 | Clayton High School | B | Pickett | Saner | Smith | Lenze | NT | Unverified: |
| 6 | Affton High School | A | NT |
