| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | ALIVERNIA, Brooklyn | 9 | Hancock High School | NT | Unverified: |
| 3 | LAHEE, Kaitlyn | 9 | Valley Park High School | NT | Unverified: |
| 5 | LOFGREN, Alexa | SR | Affton High School | 1:04.05 | Verified |
| 7 | WANG, Esther | SO | Clayton High School | NT | Unverified: |
