| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Clayton High School | B | Bernstein | Griffey | Wang | Dayal | NT | Unverified: |
| 2 | Clayton High School | A | Hopper | Hicks | Sanders | Stern | NT | Unverified: |
| 4 | Affton High School | A | Easterwood | Blackmon | Lofgren | Rolwes | 55.44 | Verified |
| 6 | Hancock High School | A | Haynes-Mcginnist | Randolph | Alivernia | Haynes-Mcginnist | NT | Unverified: |
| 8 | Bayless High School | A | NT | Unverified: |
