| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Affton High School | A | Bell | Berger | Berger | Stephens | 12:00.00 | Verified |
| 2 | Clayton High School | A | Pelligreen | Thompson | Erdmann | Riswadkar | NT | Unverified: |
| 3 | Clayton High School | B | Furdek | Wang | Mitreva | Reed | NT | Unverified: |
| 4 | Affton High School | B | NT |
