| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | St. Louis University High School | A | Mayer | Glass | Hogan | Labarge | 8:22.99 | Verified |
| 2 | Chaminade College Preparatory Sc | A | Cattoor | Hammell | Hardeman | Hogenkamp | 8:41.74 | Verified |
| 3 | DeSmet Jesuit High School | A | Weishaar | Hess | Voelker | Rasure | 8:42.78 | Verified |
| 4 | Christian Brothers College High | A | Werner | Nunley | Gunn | Gunn | 9:01.65 | Verified |
