| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | Christian Brothers College High | A | Smith | Werner | Gunn | Gunn | 3:40.49 | Verified |
| 4 | St. Louis University High School | A | Pashea | Byrne | Meehan | Wingo | 3:26.26 | Verified |
| 5 | DeSmet Jesuit High School | A | Pohl | Mosquera | Byas | Cloud | 3:36.01 | Verified |
| 6 | Chaminade College Preparatory Sc | A | Cavlovich | Cattoor | Hammell | Hogenkamp | 3:41.06 | Verified |
