| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | St. Louis University High School | A | Greiner | Lowe | Alford | Mittendorf | 3:55.20 | Unverified: SLUH JV/C #2 |
| 4 | Christian Brothers College High | A | Terrell | Wertel | Drummond | Holmes | NT | Verified |
| 5 | Chaminade College Preparatory Sc | A | Einig | Ganninger | Johnson | Nava | 3:41.06 | Verified |
| 6 | DeSmet Jesuit High School | A | Sharp | Leatherberry | Haddock | Penberthy | 4:05.00 | Unverified: |
