| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | DeSmet Jesuit High School | A | Redd | Johnson | Leatherberry | Haddock | 48.10 | Unverified: Dale Collier/Kirkwood Invitation |
| 4 | Christian Brothers College High | A | Steward | Lumkin | Van | Alford | 44.57 | Verified |
| 5 | St. Louis University High School | A | Baur | Verzino | Seal | Chaffee | 47.50 | Unverified: SLUH JV/C #2 |
| 6 | Vianney High School | A | Bradley | Curran | Roach | Kester | 52.34 | Unverified: |
