| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | Christian Brothers College High | A | Smith | Martin | Conley | Summers | 1:34.52 | Verified |
| 4 | DeSmet Jesuit High School | A | Thomas | Hentges | Cloud | Stewart | 1:32.99 | Verified |
| 5 | St. Louis University High School | A | Brooks | Bailey | Meehan | Byrne | 1:33.16 | Verified |
| 6 | Chaminade College Preparatory Sc | A | Cameron) Epps | Lee | Cavlovich | Johnson | 1:35.33 | Verified |
